The International Spy Museum is a privately owned museum dedicated to the tradecraft, history and contemporary role of espionage, featuring the largest collection of international espionage artifacts currently on public display. The museum is located within the 1875 Le Droit Building in the Penn Quarter neighborhood of Washington, D.C., and is across the street from the Old Patent Office Building (which houses the Smithsonian American Art Museum and the National Portrait Gallery). The museum is located and one block south of the Gallery Place Metro station via Red, Green and Yellow lines.

On September 23, 2013 it was announced by the Historical Society of Washington, D.C. that the International Spy Museum will be relocating to the society's Carnegie Library building at Mt. Vernon Square under a proposal that envisions a 40,000-square-foot underground addition for use by the museum, with a new glass structure on the north side of the building housing a visitor's center and other dining and entertainment venues